Fig. 9

Effect of WBH on behaviour in Shank3B- mice. A Schematic timeline of the WBH protocol and behavioural tasks undertaken (left) and the impact of that protocol on body temperature (°C) over the five hours duration of the protocol, measured by subcutaneous temperature transponders in C57 and Shank3B- mice (right). B Effect of WBH on time spent grooming across the full time course and, with individual animals shown, at 24 h after heating protocol. C Effect of WBH (5 h) on social interaction tasks: frequency of sniffs and time spent sniffing. D Effect of WBH on latency to enter the open zone and time spent on the open zone assessed by the elevated zero maze in Shank3B-, shown across time and with individual animals shown at 5 h. Data are presented as mean ± SEM (A, B, D) or median ± IQR (C and D, centre panel) (Shank3B- RT, WBH: n = 15,16, respectively, except for EPM test: n = 11,14, respectively). Data were analysed by repeated measures two-way ANOVA (A, B, D) and Kruskal–Wallis (C). Post hoc pairwise comparisons were made using Bonferroni for parametric analyses and Dunn for nonparametric analyses. Mann–Whitney U tests were performed for simple pairwise comparison of WBH and RT at 5 or 24 h. p < 0.05, 0.01 and 0.001 for WBH vs. RT group are denoted by *, ** and ***, respectively