Fig. 5From: Haploinsufficiency of autism causative gene Tbr1 impairs olfactory discrimination and neuronal activation of the olfactory system in miceReduction of inhibitory interneurons and morphological alteration of the mitral cell layer in Tbr1+/− olfactory bulbs. Double immunostaining of TBR2 and a VGLUT1, b VGLUT2, c Neurofilament-light chain (NF-LC), d Calretinin, e Parvalbumin, and f Calbindin was performed to compare olfactory bulbs of Tbr1+/− and WT mice. g Quantification of Calretinin+ interneurons at the glomerular layer (GL), external plexiform layer (EPL), mitral cell layer (MCL), and granular cell layer (GCL). h Quantification of parvalbumin+ interneurons at the external plexiform layer (EPL). i Quantification of calbindin+ interneurons at the glomerular layer (GL). Data represent mean plus SEM and the results of individual mice are shown. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars: 50 μmBack to article page