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Fig. 3 | Molecular Autism

Fig. 3

From: Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells

Fig. 3

a. OCRL and APPL-1 immunocytochemistry showing hyper-dense localization in control neuronal samples (LS200 and LS400) (arrows) compared with patient samples (LS100 and LS300). Neuronal differentiation was carried out once for each sample. All images were captured with the × 63 objective. A 10-μm bar is shown as a reference size marker. b. The immunofluorescent signals were quantified for the same area at a total of 66 soma/axon junctions for OCRL and 90 for APPL1. Differences in signal intensity were assessed between the LS samples vs their corresponding sibling controls and analyzed using a Student’s t test. One asterisk denotes a two-tailed p value of < 0.05; two asterisks indicate a p value of < 0.01 (actual values are OCRL: LS100 vs LS200 [1.35E-05]; LS300 vs LS400 [8.03E-04]. APPL1: LS100 vs LS200 [3.91E-05; LS300 vs LS400 [1.84E-02]). The error bars show the standard error of the mean

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Molecular Autism

ISSN: 2040-2392