Table 2 Stereologic parameters used for pyramidal neuron quantification in control subjects and patients with autism
From: Neuropathology of the posteroinferior occipitotemporal gyrus in children with autism
Number of sections (average) | 7.7 |
Objective 1 | 2.5× |
Objective 2 | 40× |
Disector height | 20 μm |
Guard zone | 5 μm |
Counting frame | 75 × 75 μm |
Grid size | Layer III: 750 × 750 μm |
Layer V: 500 × 500 μm | |
Layer VI: 500 × 500 μm | |
Measured thickness (μm, average) | Cut at 200 μm: 185.1 |
Cut at 600 μm: 574.8 | |
Nucleator rays | 4 |
Cavalieri grid size | 100 × 100 μm |