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Fig. 4 | Molecular Autism

Fig. 4

From: Impaired neurogenesis and neural progenitor fate choice in a human stem cell model of SETBP1 disorder

Fig. 4

SETBP1 deficiency impairs acquisition of ventral forebrain identity. A Expression of dorsal forebrain marker PAX6 (green), dorso-ventral boundary forebrain marker GHS2 (red) and ventral marker NKX2.1 (red) in day 24 cortical NPCs. Dapi was used to label all nuclei. Scale bar: 100uM. B Quantitative data presented as mean ± s.e.m for each genotype with two biological replicas per line and genotype. One-way ANOVA or Kruskal–Wallis test: PAX6 P = 0.021 (Post Hoc WT vs. SETBP1-/-, p = 0.047 WT vs. SETBP1+/- , p = 0.912; SETBP1+/- vs. SETBP1-/-, p = 0.040), NKX2.1 P = 0.003 (Post Hoc WT vs. SETBP1-/-, p = 0.221 WT vs. SETBP1+/- , p = 0.138; SETBP1+/- vs. SETBP1-/-, p = 0.003), GSH2 P = 0.006 (Post Hoc WT vs. SETBP1-/-, p = 0.011 WT vs. SETBP1+/- , p = 0.803; SETBP1+/- vs. SETBP1-/-, p = 0.022), PAX6+GSH2+/PAX6+ P = 0.032 (Post Hoc WT vs. SETBP1-/-, p = 0.022 WT vs. SETBP1 +/- , p = 0.567; SETBP1+/- vs. SETBP1-/-, p = 0.036) (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). C Expression of pan-neural forebrain marker FOXG1 (red), neural radial glia marker NES (green), dorsal forebrain marker PAX6 (red), and ventral markers NKX2.1 (green) in ventrally derived (MGE) NPCs at day 20. Dapi was used to label all nuclei. Scale bar: 100uM. D Quantitative data presented as mean ± s.e.m for each genotype with three biological replicas per line and genotype. One-way ANOVA test: NKX2.1 P = 0.038 (Post Hoc WT vs. SETBP1-/-, p = 0.042 WT vs. SETBP1+/- , p = 0.098; SETBP1+/- vs. SETBP1, p = 1), FOXG1 P = 4.77E−06 (Post Hoc WT vs. SETBP1-/-, p = 3.86E−06 WT vs. SETBP1+/- , p = 4.8E−05; SETBP1+/- vs. SETBP1-/-, p = 0.103) (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). E Schematic representation of hESC ventral forebrain (MGE) differentiation protocol to generate ventral NPC population

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