Fig. 7
Dietary zinc supplementation restores synaptic GluN1 density in the basal and lateral nuclei of the amygdala (BLA). A Representative images displaying amygdala and cortex, labelled with nuclei staining (DAPI: blue), GluN1 (green), and synapsin1/2 (red). Scale bar = 500 µm. Box 1 = example region of interest image taken in the lateral amygdala (LA) and box 2 = example region of interest image taken in the basal amygdala (BA). Scale bar = 10 µm image in the box and 5 µm for zoomed images. B Representative images of GluN1 (green) and synapsin1/2 (red) immunolabelling in the LA of WT mice and Tbr1+/− mice fed with normal or high zinc diet. C Significantly reduced synaptic density of GluN1 in Tbr1+/− LA, measured as the density of synapsin1/2-positive GluN1 puncta normalised to the WT30ppm value, is rescued by high dietary zinc. (Sample size, region of interest/animals: WT30ppm = 19/5, Tbr+/−30 ppm = 27/5, WT150ppm = 24/5, Tbr+/−150 ppm = 26/5). D Representative images of GluN1 (green) and synapsin1/2 (red) immunolabelling in the BA. E A reduction in the synaptic density of GluN1 in Tbr1+/− BA is prevented by high dietary zinc. (Sample size, region of interest/animals: WT30ppm = 20/5, Tbr+/−30 ppm = 27/5, WT150ppm = 27/5, Tbr+/−150 ppm = 28/5). Each point represents an individual region of interest image in each experimental group. Scale bar = 5 µm. All data represent mean ± standard error of the mean, analysed using one-way ANOVA with Tukey’s post hoc test. ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001