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Table 3 Experimental conditions used to perform immunofluorescence

From: Neuroglia in the autistic brain: evidence from a preclinical model

Primary antibody

Brand/cat #

Dilution

Secondary antibody

Brand/cat #

Rabbit α-GFAP

Abcam

1:200

FITC conjugated goat anti-rabbit IgG (H + L) 1:200, 5% BSA in PBS/0.25% triton X-100

Jackson ImmunoResearch

ab7260

5% BSA in PBS/0.25% triton X-100

111-095-003

Rabbit α-Olig2

Santa Cruz

1:250

FITC conjugated goat anti-rabbit IgG (H + L) 1:200, 0.5% BSA in PBS/0.25% triton X-100

Jackson ImmunoResearch

sc-48817

0.5% BSA in PBS/0.25% triton X-100

111-095-003

Rabbit α-Iba1

Wako

1:1000

FITC conjugated goat anti-rabbit IgG (H + L) 1:200, 0.5% BSA in PBS/0.25% triton X-100

Jackson ImmunoResearch

019-19741

0.5% BSA in PBS/0.25% triton X-100

111-095-003

  1. GFAP glial fibrillary acidic protein; MAP2 microtubule associated protein; BSA bovine serum albumin; FITC fluorescein isothiocyanate; PBS phosphate buffered saline