Table 2 Experimental conditions used to perform western blot experiments
From: Neuroglia in the autistic brain: evidence from a preclinical model
Primary antibody | Brand/cat # | Dilution | Secondary antibody | Brand/cat # |
|---|---|---|---|---|
Rabbit α-S100B | Genetex | 1:1000 | HRP conjugated goat anti-rabbit IgG 1:10000 | Jackson ImmunoResearch |
GTX129573 | 5% BSA in TBS-T 0.1% | 5% BSA in TBS-T 0.1% | 111-035-045 | |
Rabbit α-GFAP | Abcam | 1:25000 | HRP conjugated goat anti-rabbit IgG 1:10000 | Jackson ImmunoResearch |
ab7260 | 5% milk in TBS-T 0.1% | 5% milk in TBS-T 0.1% | 111-035-045 | |
Rabbit α-Olig2 | Santa Cruz | 1:500 | HRP conjugated goat anti-rabbit IgG 1: 10000 | Jackson ImmunoResearch |
sc-48817 | 5% milk in TBS-T 0.1% | 5% milk in TBS-T 0.1% | 111-035-045 | |
Rabbit α-CD11b | Bioss | 1:1000 | HRP conjugated goat anti-rabbit IgG 1:10000 | Jackson ImmunoResearch |
bs-1014R | 5% BSA in TBS-T 0.1% | 5% BSA in TBS-T 0.1% | 111-035-045 | |
Rabbit α-Iba1 | Abcam | 1:1000 | HRP conjugated goat anti-rabbit IgG 1:10000 | Jackson ImmunoResearch |
ab178846 | 5% milk in TBS-T 0.1% | 5% milk in TBS-T 0.1% | 111-035-045 | |
Rabbit α-β-actin | Santa Cruz | 1:1000 | HRP conjugated goat anti-rabbit IgG 1:20000 | Jackson ImmunoResearch |
sc-1616R | 5% milk in TBS-T 0.1% | 5% milk in TBS-T 0.1% | 111-035-045 |