Skip to main content
Fig. 8 | Molecular Autism

Fig. 8

From: Neuroglia in the autistic brain: evidence from a preclinical model

Fig. 8

Effect of the prenatal VPA exposure on microglia in infancy. Evaluation of the cluster of differentiation 11b (CD11b) and Iba1 in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) infant rats (PND 13). Representation of the relative concentration of CD11b (a) and Iba1 (c) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for CD11b (b) and Iba1 (d) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) (N = 3, in triplicate). Representative fluorescence micrographs of Iba1 (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) (e). The images have been analyzed by counting the number of Iba1-positive cells in 2.4 × 105 μm3 (scale bar 50 μm; N = 3, 4 times) (f). All data are presented as means ± SEM. Statistical analysis was performed by t test (*p < 0.05; **p < 0.01; ***p < 0.001 vs Veh group)

Back to article page