Fig. 2

Expression of Chd8 in the developing mouse brain. a Chd8 mRNA expression in the developing mouse brain (n = 5 at each time point) by real-time RT-PCR. The level was normalized to the E16 mice. Error bars represent SEM. b Western blot analysis of CHD8 protein using whole brain lysates at the different developmental stages of mouse brains. c Quantification of CHD8 protein levels in developing mouse brain (n = 6 at each time point) by western blot analysis that is normalized to E16 level. d Chd8 RNA in situ hybridization of sagittal and coronal sections from mouse brains at the stages indicated. Abbreviations: cortex (Ctx), lateral ventricle (LV), hippocampus (Hip), thalamus (Tha), olfactory bulb (Ob), cerebellum (Cb). Scale bar, 600 μm. e RNA in situ hybridization shows the Chd8 expression pattern in coronal sections of the cerebral cortex at the stages indicated. Scale bar, 500 μm. High levels of Chd8 mRNA are detected in the cortex at perinatal stages. Abbreviations: cortical plate (CP), ventricular zone (VZ), subventricular zone (SVZ), corpus callosum (CC). Roman numerals (I–VI) indicate layers of the cerebral cortex (scale bar, 100 μm)