Fig. 2
From: CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors
Morphological characteristics of shank3b−/− larvae and adult zebrafish. a–b Abnormal morphological changes in shank3b−/− and shank3b+/− larvae at ~ 1 dpf, including severe developmental delay, eye melanin reduction (blue arrow), and tail bending (red arrow) (+/+, N = 60; +/−, N = 50; −/−, N = 50). c–d Normal morphology and body length of shank3b+/+, shank3b+/−, and shank3b−/− larvae at 3 dpf (c) and adults (6 mpf, male) (d) (N = 20 for each genotype). e–f Significantly enlarged brain size (e) but normal brain weight (f) in adult male shank3b−/− (6 mpf) compared to WT zebrafish (N = 30 for each genotype). *p < 0.05