Fig. 4

E2 administration differently affected sociability of PV+/+, PV+/−, and PV−/− mice tested in the three-chamber assay. a Paired graphs of time spent in the chamber with the novel mouse (S) or the object (O) during the 10-min social interaction period plotted for each mouse; a negative slope (S > O) is characteristic of a “social” mouse. b Sniffing/exploration time spent close to the subject mouse, i.e., within 2 cm from the stranger/empty wired cup. c Percentage of “social” mice defined as animals with sniff duration time (S) > (O) during the 10-min test phase. d Preference index (stranger vs. object sniffing time divided by the total exploration time in the compartments containing S and O) fold change. Values close to “0” indicate no preference for the stranger mouse. Asterisks represent *p < 0.05; **p < 0.01; ***p < 0.001; n.s.: not significant. Hashtags represent significant preference for the stranger mouse over the object ^##p < 0.01; ^###p < 0.001