Fig. 3

Novel tank assay showing anxiolytic behavior in dyrk1aa KO zebrafish. a An illustration of a novel rectangular tank. Dashed lines indicate the boundaries of three vertically different zones (top, middle, and bottom). b, c Representative images of zebrafish behavior in the early time phase (1–5 min). dyrk1aa KO zebrafish display reduced anxiety-like phenotype, swimming more time in middle and top zones. d–f Duration per minute in each zone. Upon introduction to the novel tank, zebrafish display a preference to remain at the bottom zone. dyrk1aa KO fish spent significantly less times in this zone, compared to WT siblings. Black line for WT and gray line for dyrk1aa KO zebrafish. Number of fish used in this assay: n = 8 for WT fish, n = 8 for KO fish, respectively. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test