Fig. 3From: CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in cerebral organoids derived from iPS cellsValidation of selected DEGs by qPCR. The RNA samples used in the RNA-seq were used for this analysis. Samples were analyzed in triplicate as described in “Methods” section and Additional file 1: Supplemental methods. Significant differences between control and KO are denoted by an asterisk (*). The p values derived by Student’s t test were as follows: SOX2, 0.047; TCF4, 0.004; HMGA2, 0.00005; PAX6, 0.02; RELN, 0.02; CNTNAP2, 0.04; DLX6-AS1, 0.0001; MEG3, 0.00002Back to article page