Fig. 1
From: Synaptic vesicle dynamic changes in a model of fragile X
Overview of experimental design. In the mass spectrometry studies, the Fmr1 KO mouse model was compared with WT mice. Frontal cortex, hippocampal and cerebellar brain tissues were used for protein profiling using LC-MSE. For synaptosomes, both hippocampal and cerebellar tissues were used for LC-MSE and SRM-MS studies. Data analysis resulted in the identification of significant protein changes. For live-cell imaging, neurons of the Fmr1 KO mouse model were cultured and compared with WT mice for both hippocampus and cerebellum. These neurons were stained with FM1-43 dye that specifically stains synaptic vesicles. For electron microscopy, Fmr1 was specifically knocked down in Purkinje cells and these cells were used for ultrastructural analysis. Data analysis was performed in instrument specific programmes, and statistical analysis was performed using R statistical programming language