Skip to main content
Fig. 9 | Molecular Autism

Fig. 9

From: Brinp1 −/− mice exhibit autism-like behaviour, altered memory, hyperactivity and increased parvalbumin-positive cortical interneuron density

Fig. 9

Up-regulation of Astrotactin 1 and Astrotactin 2 mRNA in the embryonic brain and adult hippocampus of Brinp1 knock-out mice. a qPCR showing a significant increase in Astn1 mRNA, t(9) = 2.800, p = 0.0207, and Astn2 mRNA, t(9) = 2.829, p = 0.0222, unpaired Student’s t test, in the developing (E18.5) mouse brain. Levels of Brinp1 exon 3-deleted (Brinp1Δe3) mRNA also increase, compared to levels of Brinp1 in the WT mice: t(9) = 2.733, p = 0.0231. No significant changes overserved for Brinp2 mRNA levels t(9) = 0.4109, p = 0.6908, or Brinp3 mRNA levels: t(9) = 0.5277, p = 0.6105, unpaired Student’s t test. b An increase in Astn1 and Astn2 expression was also detectable in the hippocampus at 6 weeks of the Brinp1 −/− mice: Astn1: t (9) = 3.384, p = 0.0081, Astn2: t(9) = 2.821, p = 0.0200, unpaired Student’s t tests. No significant changes were detected in levels of exon 3-deleted (Brinp1Δe3) mRNA t(9) = 0.8505, p = 0.4171, Brinp2 mRNA t(9) = 1.616, p = 0.1405, or Brinp3 mRNA t(9) = 1.047, p = 0.3222, unpaired Student’s t tests. c In the 6-week-old Brinp1 −/− cortex, less Brinp1 exon 3-deleted (Brinp1Δe3) mRNA (t(5) = 3.611, p = 0.0154) and Brinp2 mRNA (t(5) = 3.113, p = 0.0265) were detected, with no significant change in expression of either Astn1, Astn2 or Brinp3 Astn1: t(5) = 1.572, p = 0.1909, Astn2: t(5) = 1.139, p = 0.3182, Brinp3: t(5) = 1.133, p = 0.3087, unpaired Student’s t tests. N = 3 WT, 4 Brinp1 −/−, *p < 0.05, **p < 0.01. Normalisation against GAPDH expression levels. All data represented as the mean ± SE

Back to article page