Fig. 8

Normal cell proliferation in the E18.5 Brinp1 knock-out mouse brain. a No significant change in number of Ki67+ cells (proliferation marker) in the embryonic day 18.5 (E18.5) somatosensory neocortex (coronal) of Brinp1 ^−/− mice. Neocortex: t(8) = 1.768, p = 0.1150, neocortex SVZ: t(8) = 0.2736, p = 0.7913, unpaired Student’s t tests. N = 5 WT and 5 Brinp1 ^−/− mice. b No significant change in number of Phh3+ cells (a marker of cells undergoing mitosis) in the neocortex or hippocampus of E18.5 Brinp1 ^−/− brains (coronal sections). Neocortex SVZ: t(6) = 0.1509, p = 0.8850, neocortex: t(6) = 0.04147, p = 0.9683, hippocampus SVZ: t(6) = 0.1211, p = 0.9083, hippocampus: t(6) = 2.008, p = 0.0914, unpaired Student’s t tests. N = 4 WT and 4 Brinp1 ^−/− mice. c No significant change in number of activated caspase 3-positive cells (a marker of cells undergoing apoptosis) in the neocortex or hippocampus of E18.5 Brinp1 ^−/− brain (coronal). Neocortex: t(7) = 0.2564, p = 0.8050, hippocampus: t(7) = 1.114, p = 0.3020, unpaired Student’s t tests. N = 5 WT and 4 Brinp1 ^−/−mice. d BrdU+ cell distribution in the E18.5 dentate gyrus following administration of BrdU to Brinp1 heterozygous dams at the following time points: embryonic day 12.5 (E12.5), day 14.5 (E14.5) or day 16.5 (E16.5). No significant change in cell number of E12.5-, E14.5- or E16.5-born cells (BrdU-labelled) in the E18.5 dentate gyrus. E12.5 BrdU+ cell count: t(8) = 0.7758, p = 0.4602, E14.5 BrdU+ cell count: t(8) = 0.2668, p = 0.7973, E16.5 BrdU+ cell count: t(8) = 0.01437, p = 0.9890, unpaired Student’s t test. N = 5 WT and 5 Brinp1 ^−/− mice. All images counter stained with 4′,6-diamidino-2-phenylindole (DAPI, blue)