Fig. 3

Reduced litter survival and postnatal growth of Brinp1 knock-out mice. a Breeders were monitored for litter size at birth and at age of weaning (P21). i) No significant differences in number of pups per litter at postnatal day 0, from WT × WT, het × het, Brinp1 ^−/− × Brinp1 ^−/−, or WT × Brinp1 ^−/− parents, F(3,62) = 0.1624, p = 0.9212, one-way ANOVA. ii) The knock-out allele present in breeders impacted the number of pups weaned at postnatal day 21, F(3,62) = 9.119, p < 0.001, one-way ANOVA. Tukey HSD multiple comparison tests showed significant differences: WT × WT and het × het: p = 0.122, WT × WT and Brinp1 ^−/− × Brinp1 ^−/−: p < 0.001, WT × WT and WT × Brinp1 ^−/−: p = 0.006, het × het and Brinp1 ^−/− × Brinp1 ^−/−: p = 0.018, het × het and WT × Brinp1 ^−/−: p = 0.588, Brinp1 ^−/− × Brinp1 ^−/− and WT × Brinp1 ^−/−: p = 0.377. b Brinp1 ^−/− mice weighed from week 3 to week 12. i) Female Brinp1 ^−/− mice showed a significant reduction in body weight by repeat measures two-way ANOVA: F(2,18) = 27.580, p < 0.001. A Tukey HSD multiple comparison test found female Brinp1 ^−/− mice to weight significantly less than WT littermates of the same sex (p < 0.001). Brinp1 het female mice were also found to weigh significantly less than WT (p < 0.001). No significant effect of genotype was found between female Brinp1 het and Brinp1 ^−/− mice (p = 0.362). ii) Male Brinp1 ^−/− mice also show a significant reduction in body weight by repeat measures two-way ANOVA: F(2,23) = 8.312, p = 0.002. A Tukey HSD multiple comparison test found a significant difference between male WT and Brinp1 ^−/− mice (p = 0.002) and male Brinp1 het and Brinp1 ^−/− mice (p = 0.013). No significant effect of genotype found between male WT and Brinp1 het mice (p = 0.704). Results represented as the mean ± SD *p < 0.05, **p < 0.0.1, ***p < 0.001, ****p < 0.0001