Fig. 3

Elevated expression and activity of the PI3K catalytic subunit p110δ in an autism cell line with increased pS6/S6 ratio. a Example Western blots showing increased phosphorylation of S6, increased p110δ, but no changes in S6, p110β, or tubulin in cell line A4 compared to another autism cell line (A21) and an unaffected control (CTR). b–d Densitometric quantification of pS6- (b), p110β- (c), and p110δ- (d) specific Western blots shows significantly increased S6 phosphorylation and p110δ levels but no changes in p110β (n = 4, one-way ANOVAs with Tukey’s post hoc analyses; b F(2,9) = 35.36; p < 0.0001; *p = 0.0004, ^# p < 0.0001; c F(2,9) = 0.66; p = 0.54; d F(2,9) = 10.42; p = 0.0045; *p = 0.014, ^# p = 0.0056). All values were normalized to tubulin. e p110δ-associated PI3K activity is significantly increased in cell line A4 compared to cell line A21 and the unaffected control (n = 8, one-way ANOVA with Tukey’s post hoc analyses, F(2,21) = 14.73; p = 0.0001; *p = 0.0002, ^# p = 0.0006). Bar diagrams are means + SEM