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Figure 6 | Molecular Autism

Figure 6

From: Engrailed2 modulates cerebellar granule neuron precursor proliferation, differentiation and insulin-like growth factor 1 signaling during postnatal development

Figure 6

En2 cDNA overexpression in P7 rat GNPs also elicits reduced proliferation and increased neurite outgrowth. (A) Following En2 overexpression, the BrdU LI of rat GNP at ~30 h was reduced by 67%. (B) PCNA expression was reduced 52% in P7 rat GNPs following En2 overexpression; n = 6 each vector. Data are expressed as percent PCNA + GFP+/total GFP + cells. N = 6-12 for each vector, 3 experiments. *,p ≤ 0.05. (C) Overexpression of En2 in rat GNPs doubled the proportion of cells with neuronal morphologies, replicating results observed in mouse. (D) Overexpression of En2 in rat GNPs increased the proportion of Map1b + cells among all GFP + cells, including neurite-bearing and non-neurite-bearing cells, suggesting En2 may serve to promote differentiation prior to the onset of neurite outgrowth. n = 6-15, each vector, 3–5 experiments; *, significance between GFP and En2, p ≤ 0.05.

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