Table 2 Primers used for qRT-PCR
From: Altered glial marker expression in autistic post-mortem prefrontal cortex and cerebellum
Primer name | Primer sequence (5′ to 3′) | OD | MW | % GC content | Tm(°C) |
|---|---|---|---|---|---|
ActinB-F | AGAAAATCTGGCACCACACC | 4.1 | 6064 | 50 | 60.4 |
ActinB-R | AGAGGCGTACAGGGATAGCA | 4.3 | 6240.1 | 55 | 62.4 |
Trem2-F | CCGGCTGCTCATCTTACTCT | 3.3 | 5995 | 55 | 62.4 |
Trem2-R | AGTCATAGGGGCAAGACACC | 4.2 | 6160 | 55 | 62.4 |
Dap12-F | GAGACCGAGTCGCCTTATCA | 3.8 | 6102 | 55 | 62.4 |
Dap12-R | GTCATGATTCGGGCTCATTT | 3.7 | 6114.1 | 45 | 58.4 |
Cx3cr1-F | GCAGATCCAGAGGTTCCCTT | 3.7 | 6093 | 55 | 62.4 |
Cx3cr1-R | TAACAGGCCTCAGCCAAATC | 3.9 | 6055 | 50 | 60.4 |
Gfap-F | CTGCGGCTCGATCAACTCA | 3.5 | 5748.8 | 57.9 | 62.3 |
Gfap-R | TCCAGCGACTCAATCTTCCTC | 3.6 | 6277.1 | 52.4 | 62.7 |
Nefl-F | AGCTGGAGGACAAGCAGAAC | 4.4 | 6209.1 | 55 | 62.4 |
Nefl-R | TGCCATTTCACTCTTTGTGG | 3.5 | 6065 | 45 | 58.4 |
Parvalbumin-F | CTGGAGACAAAGATGGGGAC | 4.3 | 6240.1 | 55 | 62.4 |
Parvalbumin-R | CAGAGAGGTGGAAGACCAGG | 4.4 | 6265.1 | 60 | 64.5 |
Aif1-F | AGCAGTGATGAGGATCTGCC | 4.0 | 6182.1 | 55 | 62.4 |
Aif1-R | AGCATTCGTTTCAGGGACAT | 3.9 | 6132.1 | 45 | 58.4 |