Platelets of mice heterozygous for neurobeachin, a candidate gene for autism spectrum disorder, display protein changes related to aberrant protein kinase A activity

Table 2 Differentially expressed peptides in platelets of Nbea ^+/- mice

Identification	Uniprot ID	MW (kDa)	Identified peptide	Mono-isotopic mass	Peptide sequence	Modification	Identification	WT/HET ratio	P
Thymosin β4	P20065	5.05	TYB4	4960.44	SDKPDMAEIEKFDKSKLKKTE	Acetylation	MS/MS, clustering[42] and[41]	5.2433	0.048
Thymosin β4	P20065	5.05	TYB4	4960.44	TQEKNPLPSKETIEEKQAGES	Acetylation	MS/MS, clustering[42] and[41]	5.2433	0.048
			TYB4	2150.07	SDKPDMAEIEKFDKSKLK	Acetylation	Clustering[42] and[41]	3.9551	0.024
			(1–18)	2150.07	SDKPDMAEIEKFDKSKLK	Acetylation	Clustering[42] and[41]	3.9551	0.024
			TYB4	2828.36	KTETQEKNPLPSKETIEQEKQAGES		Clustering[42] and[41]	4.4780	0.036
			(19–43)	2828.36	KTETQEKNPLPSKETIEQEKQAGES		Clustering[42] and[41]	4.4780	0.036
Talin-1	P26039	269.8	TLN	1829.88	LPAIMRSGASGPENFQVG		Clustering[42] and identified in HEK MS/MS	2.4572	0.032
Talin-1	P26039	269.8	(449–465)	1829.88	LPAIMRSGASGPENFQVG		Clustering[42] and identified in HEK MS/MS	2.4572	0.032
Transgelin-2	Q9WVA4	22.4	TAGLN2	2295.06	MGTNRGASQAGMTGYGMPRQIL		MS/MS and[41]	2.1505	0.028
Transgelin-2	Q9WVA4	22.4	(178–199)	2295.06	MGTNRGASQAGMTGYGMPRQIL		MS/MS and[41]	2.1505	0.028
Thymosin β10	Q6ZWY8	5.03	TYB10	4933.43	ADKPDMGEIASFDKAKLKKTE	Acetylation	MS/MS	2.0944	0.020
					TQEKNTLPTKETIQEKRSEIS

The molecular weight (MW) is the MW of the precursor. A positive WT/HET ratio indicates a decrease in intensity in platelets of Nbea^+/- mice. The length of the identified peptide is indicated between brackets with the numbers corresponding to the respective amino acids. Genotype differences were tested using the two-tailed t-test (n = 5 mice/genotype).

ISSN: 2040-2392