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Figure 4 | Molecular Autism

Figure 4

From: Platelets of mice heterozygous for neurobeachin, a candidate gene for autism spectrum disorder, display protein changes related to aberrant protein kinase A activity

Figure 4

Altered processing of Talin-1 and altered phosphorylation of Calpain-2. (A, B) As well as the normal levels of full-length Talin-1, the presence of the cleavage products (head and/or rod domain) was significantly reduced in platelets (A) and in brain tissue (B) of Nbea+/- mice (n = 4 samples/genotype). (C, D) Western blots of Calpain-2 and -4, responsible for Talin-1 cleavage, indicated a similar expression of both Calpain subunits in platelets (C) and in total brain lysates (D) of Nbea+/- and Nbea+/+ mice (n = 4 samples/genotype and n = 3 samples/genotype, respectively). (E) Immunoprecipitation of phospho-(Ser/Thr) protein kinase A (PKA) substrates in platelets followed by Calpain-2 western blot revealed an increase in PKA-mediated phosphorylation of Calpain-2 (n = 6 samples/genotype). (F) Immunoprecipitation of phospho-(Ser/Thr) PKA substrates in total brain tissue followed by Calpain-2 western blot showed a decrease in PKA-mediated phosphorylation of Calpain-2 (n = 4 samples/genotype).

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Molecular Autism

ISSN: 2040-2392