Table 1 Oligonucleotides used in this study
Nucleotide sequencing | Real-time PCR-based genotyping | Site-directed mutagenesis | EGFP tagging | |
|---|---|---|---|---|
Amplification | TaqMan MGB probes | |||
FWD1: TGGACTCAGCAGATCCGTCCG | FWD8: GCTCCGCCAGCTACCTGAAG | 376G-FAM: TGAGCCATGGCAGCTCC | R376G-FWD: CCTTTGTCCTGAGCCATG GCAGCTCCAGCCAGAGG | EGFP-FWD: CCGCAGGTGCACATCTTCTC |
FWD2: CTAAGCATCGCCGACCTGGT | REV7: TGGTGGGTCACGCCGTGGAT | 376R-VIC: TGAGCCATCGCAGCTCC | R376G-REV: CCTCTGGCTGGAGCTGCC ATGGCTCAGGACAAAGG | EGFP-REV: GTGGATCCCGCCGTGGATGG |
FWD3: CCGCAGGTGCACATCTTCTC | 376C-FAM: TGAGCCATTGCAGCTCC | R376C-FWD: CCTTTGTCCTGAGCCATT GCAGCTCCAGCCAGAGG | ||
FWD4: TGGAGTCTCCAGGAGTGGA | R376C-REV: CCTCTGGCTGGAGCTGCA ATGGCTCAGGACAAAGG | |||
FWD5: GTCTGGAAGTGGCTCCAGTG | ||||
REV1: CCTGGACATTCTGAGGCAGC | ||||
REV2: GATGAGCTTGACGCTGCTGAC | ||||
REV3: GTCAGCAGCGTCAAGCTCATC | ||||
REV4: CAGTCGAAGACGCCGTC | ||||
REV5: ACATGAGCAGCAGCAGG | ||||
REV6: CAGGAGCAGGATGAGAC | ||||